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engineered zymomonas mobilis tolerant to acetic acid and

engineered zymomonas mobilis tolerant to acetic acid and

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results for this questionHow are Zymomonas mobilis cells sensitive to ARTP?How are Zymomonas mobilis cells sensitive to ARTP?As shown in Fig.1 a,Z.mobilis cells were sensitive to ARTP treatment.Treatment by ARTP for 15 s resulted in a cellular death rate between 13.8 and 37.1%,and for over 30 s resulted in no cell survival.Reference biotechnologyforbiofuels.biomedcentral/articles/10.1186/s13068-0 results for this questionFeedbackEngineered Zymomonas mobilis tolerant to acetic acid and

Jan 05,2019 engineered zymomonas mobilis tolerant to acetic acid and#0183;Zymomonas mobilis mutants AQ8-1 and AC8-9 with enhanced tolerance against acetic acid were generated via a multiplex atmospheric and room temperature plasma (mARTP) mutagenesis.The growth and ethanol productivity of AQ8-1 and AC8-9 were both improved in the presence of 5.08.0 g/L acetic acid. results for this questionHow is the tolerance mechanism of Z.mobilis investigated?How is the tolerance mechanism of Z.mobilis investigated?The tolerance mechanism of Z.mobilis was investigated in terms of inhibitor degradation,cell morphology and membrane perme- ability under the stress of phenolics using GC-MS,scanning and transmission electron microscopies (SEM and TEM),as well as uorescent probes.High tolerance and physiological mechanism of Zymomonas

results for this questionWhat kind of hopanoids does Zymomonas mobilis have?What kind of hopanoids does Zymomonas mobilis have?Z.mobilis has hopanoids in its plasma membrane and this is thought to be a key to its high ethanol tolerance.Its plasma membrane has a phospholipid composition with a major component being phosphatidylethanolamine and lesser amounts of phosphatidylglycerol,cardiolipin,dimethyl phosptidylethanolamineZymomonas mobilis - microbewikiA High-Efficacy CRISPR Interference System for Gene

Nov 10,2020 engineered zymomonas mobilis tolerant to acetic acid and#0183;Zymomonas mobilis is a promising biofuel producer due to its high alcohol tolerance and streamlined metabolism that efficiently converts sugar to ethanol.Z.mobilis genes are poorly characterized relative to those of model bacteria,hampering our ability to rationally engineer the genome with pathways capable of converting sugars from plant hydrolysates into valuable biofuels and

Adaptive laboratory evolution of ethanologenic Zymomonas

Furfural and acetic acid from lignocellulosic hydrolysates are the prevalent inhibitors to Zymomonas mobilis during cellulosic ethanol production.Developing a strain tolerant to furfural or acetic acid inhibitors is difficul by using rational engineering strategies due to poor understanding of their underlyingAuthor Christiane Ribeiro Janner Herrera,Vanessa Rodrigues Vieira,Tiago Benoliel,Clara Vida Galr engineered zymomonas mobilis tolerant to acetic acid and#227;o Corr engineered zymomonas mobilis tolerant to acetic acid and#234;Publish Year 2021Paradigm for industrial strain improvement identifies Jun 08,2010 engineered zymomonas mobilis tolerant to acetic acid and#0183;Other recombinant strains have been engineered to ferment xylose,arabinose,and other substrates with high yields,but low tolerance to acetic acid and decreased ethanol tolerance have been reported (10,1719).Acetic acid is an important inhibitor produced by the deacetylation of hemicelluloses during biomass pretreatment.

Cited by 120Publish Year 2010Author Shihui Yang,Miriam L.Land,Dawn M.Klingeman,Dale A.Pelletier,Tse-Yuan S.Lu,Stanton L.MartinEstimated Reading Time 13 minsInsights into Acetate Toxicity in Zymomonas mobilis 8b

Sep 30,2014 engineered zymomonas mobilis tolerant to acetic acid and#0183;Engineered Zymomonas mobilis tolerant to acetic acid and low pH via multiplex atmospheric and room temperature plasma mutagenesis journal,January 2019.Wu,Bo; Qin,Han; Yang,Yiwei; Biotechnology for Biofuels,Vol.12,Issue 1; DOI 10.1186/s13068-018-1348-9Cited by 16Publish Year 2019Author Weiting Wang,Bo Wu,Han Qin,Panting Liu,Yao Qin,Guowei Duan,Guoquan Hu,Mingxiong HeEstimated Reading Time 9 minsAdaptive laboratory evolution of ethanologenic Zymomonas May 03,2015 engineered zymomonas mobilis tolerant to acetic acid and#0183;An adaptive mutation procedure combination of NTG mutagenesis was first developed for screening of acetic acid-tolerant Z.mobilis,and a desired evolved mutant (ZM6014) obtained (Wang 2008).In this study,we also successfully obtained a novel acetic acid-tolerant Z.mobilis A7-2 without any previous mutagenesis.

Cited by 20Publish Year 2017Author Yu-Fan Liu,Chia-Wen Hsieh,Yao-Sheng Chang,Being-Sun WungEstimated Reading Time 10 minsFlocculating Zymomonas mobilis is a promising host to be

In addition,tolerance to inhibitory byproducts released during biomass pretreatment,particularly acetic acid and vanillin,was improved.These experimental results indicate that Z.mobilis,particularly its flocculating strain,is superior to S.cerevisiae as a host to be engineered for fuel ethanol production from lignocellulosic biomass.Cited by 20Publish Year 2019Author Bo Wu,Han Qin,Yiwei Yang,Guowei Duan,Shihui Yang,Fengxue Xin,Chunyan Zhao,Huanhuan Shao,Yanw(PDF) Engineered Zymomonas mobilis tolerant to acetic acid Results Zymomonas mobilis mutants AQ8-1 and AC8-9 with enhanced tolerance against acetic acid were generated via a multiplex atmospheric and room temperature plasma (mARTP) mutagenesis.The

Cited by 20Publish Year 2019Author Bo Wu,Han Qin,Yiwei Yang,Guowei Duan,Shihui Yang,Fengxue Xin,Chunyan Zhao,Huanhuan Shao,YanwEngineered Zymomonas mobilis tolerant to acetic acid and

Jan 05,2019 engineered zymomonas mobilis tolerant to acetic acid and#0183;Zymomonas mobilis mutants AQ8-1 and AC8-9 with enhanced tolerance against acetic acid were generated via a multiplex atmospheric and room temperature plasma (mARTP) mutagenesis.The growth and ethanol productivity of AQ8-1 and AC8-9 were both improved in the presence of 5.0-8.0 g/L acetic acid.Cited by 20Publish Year 2019Author Bo Wu,Han Qin,Yiwei Yang,Guowei Duan,Shihui Yang,Fengxue Xin,Chunyan Zhao,Huanhuan Shao,YanwEstimated Reading Time 9 minsEngineered Zymomonas mobilis tolerant to acetic acid and Microbial mutants able to tolerate high concentration of acetic acid are needed urgently to alleviate this inhibition.Results Zymomonas mobilis mutants AQ8-1 and AC8-9 with enhanced tolerance against acetic acid were generated via a multiplex atmospheric and room temperature plasma (mARTP) mutagenesis.The growth and ethanol productivity of AQ8-1 and AC8-9 were both improved in the presence of 5.0-8.0 g/L acetic acid.

Cited by 28Publish Year 2014Author Ning Zhao,Yun Bai,Chen-Guang Liu,Xin-Qing Zhao,Jian-Feng Xu,Feng-Wu Bai,Feng-Wu BaiEffect of acetic acid on ethanol production by

by Zymomonas mobilis mutant strains through continuous adaptation Yu-Fan Liu1,2,Chia-Wen Hsieh3*,Yao-Sheng Chang3 and Being-Sun Wung3 Abstract Background Acetic acid is a predominant by-product of lignocellulosic biofuel process,which inhibits microbial biocatalysts.Development of bacterial strains that are tolerant to acetic acid is Cited by 2Publish Year 2020Author Hoda Nouri,Hamid Moghimi,Sayed-Amir Marashi,Elahe ElahiEstimated Reading Time 10 minsHigh tolerance and physiological mechanism ofexisting in corncob residue (2-furoic acid,p-hydroxybenzoic acid,p-coumaric acid,vanillic acid,ferulic acid,and syringic acid).The tolerance mechanism of Z.mobilis was investigated in terms of inhibitor degradation,cell morphology and membrane perme-ability

Cited by 39Publish Year 2019Author Juan Xia,Yongfu Yang,Chen-Guang Liu,Shihui Yang,Feng-Wu BaiIncreased salt tolerance in Zymomonas mobilis strain

Jul 20,2020 engineered zymomonas mobilis tolerant to acetic acid and#0183;Wu B,Qin H,Yang Y,Duan G,Yang S,Xin F,Zhao C,Shao H,Wang Y,Zhu Q,et al.Engineered Zymomonas mobilis tolerant to acetic acid and low pH via multiplex atmospheric and room temperature plasma mutagenesis.Biotechnol Biofuels.2019;12(1):10.PubMed PubMed Central Article Google Scholar 31.Cited by 39Publish Year 2019Author Juan Xia,Yongfu Yang,Chen-Guang Liu,Shihui Yang,Feng-Wu BaiProgress and perspectives on developing ZymomonasJun 29,2021 engineered zymomonas mobilis tolerant to acetic acid and#0183;Abstract Zymomonas mobilis,the only microorganism known to use the Entner-Doudoroff (ED) pathway anaerobically,can produce ethanol naturally from glucose,fructose and sucrose with many desirable traits such as ethanol production at high rate and yield and merit with biosafety (generally regarded as safe,GRAS),which has attracted more attention to be engineered as cell

Cited by 3Publish Year 2020Author Katsuya Fuchino,Per BruheimEstimated Reading Time 10 minsFlocculating Zymomonas mobilis is a promising host to be

Zymomonas mobilis is superior to Saccharomyces cerevisiae as a host to be engineered for bioethanol production from lignocellulosic biomass,due to improved ethanol yield associated with its unique ECited by 51Publish Year 2015Author Zong-Xia Shui,Han Qin,Bo Wu,Zhi-yong Ruan,Lu-shang Wang,Fu-Rong Tan,Jing-Li Wang,Xiao-Yu TangEstimated Reading Time 11 minsMetabolic engineering of Zymomonas moblis for ethylene Jan 29,2021 engineered zymomonas mobilis tolerant to acetic acid and#0183;In this study,Zymomonas mobilis has been engineered to produce ethylene through the introduction of the synthetic ethylene-forming enzyme (EFE).We also investigated the effect of systematically knocking out the competitive metabolic pathway of pyruvate in an effort to improve the availability of pyruvate for ethylene production in Z.mobilis expressing EFE.

Cited by 51Publish Year 2015Author Zong-Xia Shui,Han Qin,Bo Wu,Zhi-yong Ruan,Lu-shang Wang,Fu-Rong Tan,Jing-Li Wang,Xiao-Yu TangInsights into acetate toxicity in Zymomonas mobilis 8b

An engineered Z.mobilis strain 8b was constructed expressing heterologous genes of talB,tktA,and xylAB for xylose utilization as well as truncating the endogenous lactate dehydrogenase gene ldh for improved flux to ethanol.Z.mobilis 8b is more sensitive to acetate when grown in xylose.Effect of acetic acid on ethanol production by Zymomonas Aug 01,2017 engineered zymomonas mobilis tolerant to acetic acid and#0183;Engineered Zymomonas mobilis tolerant to acetic acid and low pH via multiplex atmospheric and room temperature plasma mutagenesis.Wu B,Qin H,Yang Y,Duan G,Yang S,Xin F,Zhao C,Shao H,Wang Y,Zhu Q,Tan F,Hu G,He M.Biotechnol Biofuels,12:10,05 Jan 2019

Effect of acetic acid on ethanol production by Zymomonas

Aug 01,2017 engineered zymomonas mobilis tolerant to acetic acid and#0183;The acetate- tolerant mutant of Z.mobilis was selected and mutated with NTG.1 mL of NTG solutions (0.4 mg/mL) were added to 9 mL of the cell suspension of ZM481.The mixture was incubated at 37 engineered zymomonas mobilis tolerant to acetic acid and#176;C and agitated at 200 rpm for 30 and 45 min,Engineering Zymomonas mobilis for Robust Cellulosic Sep 01,2019 engineered zymomonas mobilis tolerant to acetic acid and#0183;When ethanol fermentation was performed with Z.mobilis ZM4 and its self-flocculating mutant Z.mobilis ZM401 by using medium supplemented with furfural,hydroxymethyl furfural (HMF),acetic acid,and vanillin,respectively,improved tolerance to acetic acid and vanillin was observed for the mutant and consequently more ethanol was produced .

Engineering Zymomonas mobilis for Robust Cellulosic

When ethanol fermentation was performed with Z.mobilis ZM4 and its self-flocculating mutant Z.mobilis ZM401 by using medium supplemented with furfural,hydroxymethyl furfural (HMF),acetic acid,and vanillin,respectively,improved tolerance to acetic acid and vanillin was observed for the mutant and consequently more ethanol was producedEngineering Zymomonas mobilis for the Production of Jun 24,2021 engineered zymomonas mobilis tolerant to acetic acid and#0183;microorganisms Article Engineering Zymomonas mobilis for the Production of Xylonic Acid from Sugarcane Bagasse Hydrolysate Christiane Ribeiro Janner Herrera 1,Vanessa Rodrigues Vieira 1,Tiago Benoliel 1,Clara Vida Galr engineered zymomonas mobilis tolerant to acetic acid and#227;o Corr engineered zymomonas mobilis tolerant to acetic acid and#234;a Carneiro 1,2,Janice Lisboa De Marco 1,L engineered zymomonas mobilis tolerant to acetic acid and#237;dia Maria Pepe de Moraes 1,Jo engineered zymomonas mobilis tolerant to acetic acid and#227;o Ricardo Moreira de Almeida 2 and Fernando Araripe Gon engineered zymomonas mobilis tolerant to acetic acid and#231;alves Torres 1,*

Enhancement of acid tolerance in Zymomonas mobilis by a

Expression of Pbp was confirmed in both JM105 and CP4 by HPLC.Z.mobilis CP4 carrying pJB99-2 (Pbp) exhibited increased acid tolerance (p engineered zymomonas mobilis tolerant to acetic acid andlt; 0.05) in acidified TSB (HCl [pH 3.0] or acetic acid [pH 3.5]),glycine-HCl buffer (pH 3.0),and sodium acetate-acetic acid buffer (pH 3.5) in comparison to the parent strain (CP4) and CP4 with pJB99 Estimated Reading Time 7 minsRESEARCH Open Access Engineered Zymomonas mobilisResults Zymomonas mobilis mutants AQ8-1 and AC8-9 with enhanced tolerance against acetic acid were generated via a multiplex atmospheric and room temperature plasma (mARTP) mutagenesis.

Ethanol fermentation technology Zymomonas mobilis

Biochemistry of Zymomonas mobilis aldehyde dehydrogenase to oxidize acetaldehyde to acetic acid.Adh mutants of Z.mobilis showing increased levels of acetaldehyde production have been isolated by using In Z.mobilis,D-glucose and o-fructose are transported by a ,a,coho,as a sdective agenti4 facilitated diffusion .Flocculating Zymomonas mobilis is a promising host to be Nov 06,2013 engineered zymomonas mobilis tolerant to acetic acid and#0183;In addition,tolerance to inhibitory byproducts released during biomass pretreatment,particularly acetic acid and vanillin,was improved.These experimental results indicate that Z.mobilis,particularly its flocculating strain,is superior to S.cerevisiae as a host to be engineered for fuel ethanol production from lignocellulosic biomass.

Genome shuffling enhances stress tolerance of Zymomonas

Dec 16,2019 engineered zymomonas mobilis tolerant to acetic acid and#0183;Rational modifications and random mutagenesis have been applied to enhance acetic acid tolerance in Z.mobilis.Expression of the exogenous gene Pbp,a 24-amino acid proton-buffering peptide,could improve the transient tolerance of Z.mobilis CP4 to low pH and acids .Images of Engineered Zymomonas mobilis tolerant to acetic imagesEngineered Zymomonas mobilis tolerant to acetic acid and Results Zymomonas mobilis mutants AQ8-1 and AC8-9 with enhanced tolerance against acetic acid were generated via a multiplex atmospheric and room temperature plasma (mARTP)

Impact of hfq and sigE on the tolerance of Zymomonas

Oct 09,2020 engineered zymomonas mobilis tolerant to acetic acid and#0183;mobilis,AcR (acetic acid resistant),showed that the sodium-proton antiporter gene nhaA (ZMO0117) plays a critical role in acetic acid resistance [13,34].Our results demonstrated than in recombinant strains ZM4-sigE and ZM4-hfq with a high content of regulatory factors SigE and Hfq protein,the expression level of nha A was increased 4.4 and 3.13-fold,respectively.Molecular Mechanism of Engineered Zymomonas Mobilis Backgroud Acetic acid and furfural are two major inhibitors during lignocellulosic ethanol production.In our previous study,we successfully constructed an engineered Zymomonas mobilis ZM532 strain tolerant these double inhibitors by genome shuffling,but the molecular mechanisms of tolerance to these inhibitors are still unknown.This study investigated the responses of ZM532 and wild-type

Open fermentative production of fuel ethanol from food

).Proteomic and Metabolomic Analysis of the Cellular Results Quantitative proteomics and metabolomics approaches were used to determine the cellular responses of Z.mobilis ZM4 to representative biomass-derived inhibitors like formic acid,acetic acid,furfural,5-hydroxymethylfurfural,and phenol.The differentially expressed proteins identified under the challenge of single and combined inhibitors were involved in cell wall/membrane biogenesis,energy

Some results are removed in response to a notice of local law requirement.For more information,please see here.12345NextZymomonasmobilismutants AQ8-1 and AC8-9 with enhanced toleranceagainst aceticacidwere generated via a multiplex atmospheric and room temperature plasma (mARTP) mutagenesis.The growth and ethanol productivity of AQ8-1 and AC8-9 were both improved in the presence of 5.08.0 g/L aceticacid.Cited by:20Publish Year:2019Engineered Zymomonas mobilis tolerant to acetic acid and

Was this helpful?People also askWhy is Zymomonas mobilis used for ethanol production?Why is Zymomonas mobilis used for ethanol production?Researchers are investigating ethanol production from cellulosic materials by genetically engineered Zymomonas mobilis.Because of its inability to naturally metabolize complex carbohydrates strains are being engineered with the purpose of being able to ferment complex carbohydrates.Zymomonas mobilis - microbewiki


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